Biochemistry

During the interaction of glyceraldehyde-3-phosphate with a special enzyme - HSE, in the protein part of which is a sulfhydryl group (SH) contained, intermediate enzyme-substrate complex forms'. HC = O 1 H — c — OH + HS-enzyme 1 H 2 C — 0 — PO 3 H 2 S — enzyme 0 — PO 3 H 2 I H Glyceraldehyde-3-phosphate Enzyme-substrate complex Then its dehydration with the participation of NAD happens, and herein a restoredformofNADFf and an intermediate high-energy complex appear. OH I H — C — S — ■ enzyme I H — С-ОН + НАД I H 2 C — 0 — PO 3 H 2 glyceraldehyde phosphate dehydrogenase _________ 0 // H — C ~ S — enzyme I H — С — OH + НАД -H(+ H+) I H 2 C — 0 — PO 3 H 2 Enzyme-substrate complex Intermediate high-energy compound When interacting with inorganic phosphoric acid, 1 ,3-diphosphoglycerol acid is formed. 0 H — C ~ S — enzyme I H — C — OH + HS — enzyme I H 2 C — О — PO 3 H 2 О С — О ~ PO 3 H 2 I — ... > H — C — OH + HS — enzyme * I H 2 C — 0 — P0 3 H 2 Intermediate compound high-energy 1,3 Diphosphoglycerate • (1,3-diphosphoglycerol acid) After that, a high-energy compound interacts with ADP, as a result of which ATP and 3-phosphoglycerate are formed. 223